Journal article
Hydrogel oxygen reservoirs increase functional integration of neural stem cell grafts by meeting metabolic demands
Y Wang, ER Zoneff, JW Thomas, N Hong, LL Tan, DJ McGillivray, AW Perriman, KCL Law, LH Thompson, N Moriarty, CL Parish, RJ Williams, CJ Jackson, DR Nisbet
Nature Communications | Published : 2023
Abstract
Injectable biomimetic hydrogels have great potential for use in regenerative medicine as cellular delivery vectors. However, they can suffer from issues relating to hypoxia, including poor cell survival, differentiation, and functional integration owing to the lack of an established vascular network. Here we engineer a hybrid myoglobin:peptide hydrogel that can concomitantly deliver stem cells and oxygen to the brain to support engraftment until vascularisation can occur naturally. We show that this hybrid hydrogel can modulate cell fate specification within progenitor cell grafts, resulting in a significant increase in neuronal differentiation. We find that the addition of myoglobin to the ..
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Grants
Awarded by Australian Research Council
Funding Acknowledgements
We would like to acknowledge the generous gift from Mr. Tom Barbieri and Mrs. Vivienne Barbieri that has contributed towards this study. This is greatly appreciated. Access to the facilities of the Centre for Advanced Microscopy (CAM) with funding through the Australian Microscopy and Microanalysis Research Facility (AMMRF) is gratefully acknowledged. This work was funded by an NHMRC Ideas Grant GNT 1185094 to D.R.N. and C.J.J. and an Australian Research Council Discovery Grant DP220102549 to D.R.N. and C.J.J.